In Vitro Response of Encapsulated Shoot Tips of Spilanthes Acmella

Multiple shoots were achieved through axillary bud explant of Spilanthes acmella on MS medium supplemented with 0.5mg/l BA. In vitro developed axillary buds and tiny shoots isolated from the multiple shoots were encapsulated in different strength of sodium alginate. The present work explains the role of components of culture medium on morphogenic response of encapsulated shoot tips to various types of growth regulators in different concentrations, MS strength on storage time and percentage of sodium alginate. The development of shoots from the encapsulated shoot tips were investigated and the maximum response (92 %) for growth ability of encapsulated buds into plantlets were achieved on MS medium supplemented with 1.0mg/l BAP, after 2 to 4 weeks of culture. The percentage of development of shoots from the encapsulated shoot buds of synthetic seeds into plantlets was affected by concentration of growth regulators and storage time and the type of media. Maximum growth was recorded in MS medium than Nitsch and B 5 media. Maximum response was obtained in 3% of sodium alginate. Synthetic seeds were stored at 4oC that remained viable for 12weeks.